New publication online: S. Johann et al.
Johann, Sarah; Goßen, Mira; Behnisch, Peter A., Hollert, Henner; Seiler, Thomas-Benjamin
Combining Different In Vitro Bioassays to Evaluate Genotoxicity of Water-Accommodated
Fractions from Petroleum Products
Content: Optimization and evaluation of small-scale higher-throughput assays (Ames fluctuation, micronucleus, Nrf2-CALUX®) as first screening tools for genotoxicity assessment of oils. Significantly increased chromosomal aberrations in zebrafish liver (ZF-L) cells were observed. Oxidative stress was not correlated with observed DNA damage. Application of a chemical dispersant increased the genotoxic potential by the increased bioavailability of dissolved and particulate oil compounds. Results show that the combination of different in vitro assays is important. The ZF-L capable of active metabolism and DNA repair seems to be a promising model for WAF testing.
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